Hi,
I’m trying to estimate the content of phytate in seeds.
A lot of articles use the colorimetric using BAPNA and Wade reagent. When I tried to use it, but it was unreliable (especially due to the low concentrations of phytate in pea seed- 0.2-0.8 g/100g). Did someone else had problems with the assay? Did you encounter problems with the fact that the assay measure iron and not directly phytate? Because there are other things in the seed and especially in the seed coat that bind iron.
I’m trying to find an easy and fast method, so doing anion-exchange purification is not an option.
I would be happy to hear any idea.
Thanks
Yemima
Dear Yemima givaty rapp
Please also see the attached article.
Regards
Kazem Ghassemi-Golezani
Thank you so much.
I’m aware to the problems testing phytate and the complexes it may create, but I’m trying to find a way to overcome this problem using the wade reagent method. I already bought all the reagents and also there isn’t any other way that is so simple and cheap. It’s the only way fitted for screening… also, some articles used this test for testing phytate, and did it without ion exchange purification, so I’m trying to understand if they also encountered problems.
Thanks again
you could use https://secure.megazyme.com/files/Booklet/K-PHYT_DATA.pdf
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