I don't have an eyepiece with Grid, so I use the software Image J to calculate the volume of various particles (eg: Seminiferous tubules ST) by two formulas, I like that you give me your opinion which is more fair:
1) according to Solari (1973)
V ° = P ° / P x V
which
V °: the volume of seminiferous tubules
P ° the number of grid points set by Image J, which overlap with the desired particle
P: the total number of lattice points
V: the total volume of the organ (testis) after fixation
2) Delesse (1884) and Glagolev (1933)
V° = Vr x (M / m) × C
which:
V °: the volume of seminiferous tubules
Vr= P ° / P (as explained above)
M: the mass of the body after fixation
m: the density of the body after fixation (≈ 1.025 according to the same authors)
C: coefficient of shrinkage of the testicular parenchyma after fixation (≈ 0.7 also by the same authors)
thank you that you took the time to read my message and thank you in advance for your reply.
Djallel Eddine.
that is a great idea. i worked on something similar, although it sounded fictitious, but it yielded positivity, best of luck
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