Dear all
In my study I want to measure Urease and phosphatase activity, I am asking about the time of the measurement for these enzymes, during growing season or after crop harvest. And which are the best methods for measurement?
Thanks
Please check the following link and PDF attachments.
https://www.researchgate.net/publication/232903992_Determination_of_soil_phosphatase_activity_using_a_microplate_method_1
Article Determination of soil phosphatase activity using a microplate method 1
Mohammed,
Sampling time depends on the objective of your project. If you are monitoring potential plant available N and P from mineralization, then you may wish to sample in spring and later near harvest - this should give an idea of nutrient release during season. otherwise, if this is a one-time sampling, suggest either spring or fall when soil is at about average annual temperature.
For determination ..apart from the above answers, you may refer method given by Eivazi and Tabatabai, Kendeler and Gerber
The best time for measurement of urease and phosphatase activities are at critical growth stage of the plants , that is between 6 and 8 weeks of different plant growth period where you may get maximum activity of these soil enzymes.
Regarding methods we found Douglas and Bremner (1970) for Urease estimation and Tabatabai and Bremner (1969) for phosphatase estimation are reliable methods.
Also go through the paper..available here:
https://pubag.nal.usda.gov/pubag/downloadPDF.xhtml?id=61980&content=PDF
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