How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

No detection in Immunofluorescence Staining of control while no much difference in the both groups in western blot, why?

23 November 2020 8,529 3 View

What online teaching new topics to address as a phd topic?

Greetings, I have just started my phd in the field of english language studies, Im currently interested in online teaching, e-learning but mostly english language pedagogy. what are the new...

16 November 2020 4,045 9 View

Hi There any one can help on the case study below?

04 October 2020 7,234 1 View

Best strategy to transfect large genes in mammalian cells?

I have an insert ( ORF 6,9kb long) that I want to transfect in a human colon cancer cell line. I initially wanted to do with lentivirus, since I could have a stable expression of my gene and...

22 April 2020 3,277 3 View

What to do with coincident features for Geographically weighted regression?

I am sorry if this is a simple question. I am trying to conduct a GWR for hedonic analysis of hotel room rates. For OLS I have conducted analysis including different room types (e.g. double,...

16 April 2020 7,062 3 View

Can a conjugated isotype antibody be used in cell culture function blocking antibody experiment?

I have a PE conjugated mouse k isotype antibody..I would like to use it as control and add to cell culture .I would like to know whether conjugate will interfere in cell culture...

22 January 2020 607 2 View

How to find the thickness of graphene nanosheets by the AFM images?

i have seen many reports have used AFM images to find the thickness of graphene and graphene like structures. Is there any specific software or method to find the thickness of the Sheets.

14 January 2020 9,982 3 View

Why the BET Surface Area decreases with increasing carbonization Temperature?

I have prepared Carbon frameworks at 700 and 800(degree C) temperatures. The Surface area obtained for 700 (degree C) is higher than 800 (degree C).

01 January 2020 6,471 5 View

What should be a loading control in condition media for zymography?

I want to do a zymography in condition media from cancer cell line...I would like to know what should be a loading control for condition media specific for zymography? Whether loading control can...

13 November 2019 1,697 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View