Why do my cells die during transfection with JetPei?

Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...

03 February 2021 1,296 6 View

Why was sonication unsuccessful for 1 of 6 samples?

I have sonicated six HeLa-cell samples in RIPA lysis buffer (prior inc. on ice, 15 min). For one of these samples, the sonication was unsuccessful. My question is what factors should be taken into...

17 December 2020 8,531 2 View

Maskning for polysterene substrate?

We want to deposite a fine pattern ( 100-300 nm thick and 10 microns wide pattern) of Pt as temperature sensors on a polysterene substrate with a glass temperature of 95 degrees C. How would...

12 November 2020 9,761 3 View

Someone recommend to use CFSE to stain parasites as Trypanosoma or Leishmania species to detect cells infection?

I have seen that some groups use CSFE to stain some parasite species before infect cells to detect percetage of infected cells. Could I use CSFE to stain Trypanosoma cruzi and measure infection in...

15 August 2020 1,898 2 View

Barriers and Factors with wound are discharge planning, how to narrow down this topic?

I'm DNP student that wants to discuss wound care discharge planning for my research. This is a very broad topic, and needing help to narrow the topic down. Any ideas on wounds that need more...

29 May 2020 8,870 1 View

Why are some plasmids (such as pKD46) temperature sensitive? What happens at higher temperatures to cause plasmid loss?

I am trying to figure out how temperature sensitive plasmids are made/isolated and what the biochemical/cellular mechanism is that causes them to be lost/lose function at higher temperatures.

20 March 2020 6,815 3 View

In 6-line Ferrihydrite synthesis is dialysis necessary or can the salt be removed with washing?

I don't have dialysis tubing and am wondering if washing is sufficient.

09 March 2020 5,484 3 View

What would be the best method to attach a radio transmitter to a grey-headed robin?

I am planning to use radio telemetry in a study of grey-headed robins (Heteromyias cinereifrons). These birds weigh around 35 g and I have sourced glue-on transmitters weighing 0.9 g (

09 December 2019 3,623 3 View

Where can I find the KIDCOPE sacles for both children and adolescence, along with scoring guidance?

The KIDCOPE scales are widely used to measuring coping in children and adolescents. There is a lot of literature using them, or assessing them, but nowhere can I find the actual scales and...

03 December 2019 4,451 2 View

How do I make a map in QGIS of a area to get GPS coordinate when no signal is available?

Th question above explains most of what I am looking for. I have been given a great opportunity for a field project but I am only just now learning of QGIS and I have a bit of a time constraint....

08 October 2019 2,389 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View