I am working on Natural product Chemistry and I am interested to test my natural products against HIV/AIDS. The best and easy choice would be to do some in vitro testing first. That's why I am interested to get some info. Looking forward to get your kind response please.
One easier in-vitro assay of the determining anti-HIV activity.
Investigation of the viral activity of compounds
In order to assess the anti-HIV-1 activity, a solution of the analyzed substances in water or dimethylsulfoxide (DMSO, the final concentration in the medium was no higher than 0.1%), was added to the cells; after 2–8 h (depending on the inhibitor), the cells were infected with pseudo-HIV-1 particles. The relative level of infection was determined by flow cytofluorimetry on an Epics 4XL Beckman Coulter instrument (USA) 48 h following the infection.
Article Screening of Potential HIV-1 Inhibitors/Replication Blockers...
Dear Shazia
Have a look to the below publications
Article Exploration of Phytochemicals Found in Terminalia sp. and th...
Article Natural Products as Anti-HIV Agents and Role in HIV-Associat...
https://www.ejmanager.com/mnstemps/82/82-1434471677.pdf?t=1557223612
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