Can publishing a prepublished research as a new book chapter be acceptable in the academic world?

Research papers are in brief, book chapters are in detail. Can publishing a prepublished research as a new expanded book chapter be acceptable in the academic world?

19 March 2020 6,221 9 View

Why does the universe expand despite self-gravity?

We are in an expanding Universe as per the big-bang cosmology. Does it really mean that the outward thermal pressure now beats the inward gravitational pressure in the Universe? What is the exact...

14 March 2020 4,196 37 View

What are the different projects launched with a strong mission to make knowledge globally free?

What are the different projects launched with a strong mission to make knowledge and its resources globally free so that unprivileged and underprivileged scholars could pursue higher education in...

28 December 2019 9,526 3 View

How can we prevent ear-problems often encountered by passengers after a flight journey?

The vertical atmospheric air pressure differences encountered during flight journeys often create ear-problems well realized by passengers after landing. How can we prevent them easily?

26 November 2019 9,015 12 View

What is the significance of dynamical system analysis in cosmology and dark energy study??

What is the reason that we modify our system as dynamical systems while studying dark energy? And further, what is the criteria for choice of the dimensionless variables x,y,z.??

29 September 2019 7,687 1 View

How to get low energy model Hamiltonian of a material from DFT calculation?

I need to find the low energy model Hamiltonian for a material (semimetal). I heard that this is possible by using Wannier90 clubbed with VASP or QE. It would be very helpful for me if someone...

18 September 2019 6,649 2 View

What are the additional advantages that a Fano resonance possesses in comparison to normal dipolar resonance in Optical systems?

Fano resonance is a distinctive spectroscopic behavior in many optical systems. There're certain differences in terms of physics and behavior of Fano resonance compare to dipole resonance which is...

30 May 2019 4,244 2 View

What is the best process for estimating Triclosan from sediment samples?

What is the best and easiest protocol for extracting Triclosan from sediment and further estimation using HPLC, GC-MS or LC-MS? Please suggest some easy and dependable protocol.

15 March 2019 2,007 4 View

Do electrons and protons have independent minds to decide the directionality of gyromagnetic effects?

We have mind. We are made up of matter. Does matter have its own mind? Do electrons and protons, which are the building blocks of matter, have independent minds to decide the directionality of...

25 February 2019 2,405 3 View

Will any expert recruit me as a postdoc under his/her supervision in the field of bioenergy or waste water treatment? Will you please share this?

I, Dr. Rachan Karmakar, have done my Ph.D. in 2018 in the field of energy and environment. My areas of specialization are as follows- 1. Biofuel production and characterization 2. Analysis of...

15 January 2019 9,065 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View