How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

What is the effect of room temperature on brain tissue intended for single nuclei RNA sequencing?

I am planning to do single-nuclei sequencing for brain organoids. However, the organoids experienced 25 minutes in room temperature prior to freezing. Most of the time they were in their culture...

17 January 2021 5,257 1 View

Why are the organoid cells showing blue fluorescence even without dapi stain?

Mouse intestinal organoids ; After fixation and storage in PBS-T, when I checked in microscope, the organoid show fluorescent color blue even if I have not added DAPI. Does anyone have such...

05 January 2021 2,067 3 View

Why monoethanol amine is used as flocculant?

I want to know the reaction mechanism of monoethanol amine used as flocculating agent for rerefining used lubricating oil. Here I have attached one research paper where MEA (monoethanolamine) is...

01 January 2021 5,681 2 View

How do you fix organoids for IHC staining and H&E sections??

I work with ovarian cancer cell line organoids embedded in matrigel, they grow well the A2780 cells, but once I start using 10% formaldehyde to fix them they disintegrate...does anybody have a...

29 November 2020 1,900 1 View

What is the best way to get organoids of the same size?

I would like to get organoids of the same size. We filter organoids using a 100 um filter and then pass that filtrate through a 40 um filter followed by flipping the 40 um filter. Then we wash the...

22 November 2020 9,391 3 View

Is this yeast contamination and how do I get rid of it?

I received this gastric tumor biopsis and processed using standard protocol to establish gastric cancer organoids. Contamination looks like this. I have never seen this before and the culture...

08 November 2020 8,541 4 View

How to simulate motion of microneedle into tissue in COMSOL?

Hi, I am new to COMSOL and need to simulate the microneedle penetrating the tissue. I have both my tissue and probe design ready in solid mechanics. How do I add prescribed velocity to the...

14 October 2020 3,823 3 View

How to successfully produce agarose microwells with PDMS stamp?

05 October 2020 3,696 3 View

Is it possible for a gas absorption reaction be first diffusion-controlled at low loading, then turns kinetic-controlled?

It's about CO2 absorption with MEA in miniscule volume. And would a pseudo first order reaction assumption still be valid if there is no mixing and agitation?

19 September 2020 1,616 1 View