What are the most important explanatory assets ?

06 December 2020 5,474 2 View

What are the methods of writing the interpretation ?

06 December 2020 3,267 3 View

Cell culture techniques: how to deactivate a viral infection while preserving cytokine storm markers?

Hello, I am trying to measure the cytokine storm markers following SARS-Cov-2 viral infection of lung cells and macrophages under different conditions. However, finding a safe way to deactivate...

05 November 2020 1,174 4 View

How can I calculate the am ount required to prepare the following buffer?

ammonium sulfate 0.17 M - polyethylene glycol 4000 25.5% (w/v) - glycerol 15% solution How can I calculate the amount required to prepare the following buffer? I want to prepare 1000 microliter

03 October 2020 4,018 5 View

Why some viral proteins are unable to express and purify in pET28a expression and purification system?

Dear All, I am trying to clone and purify codon-optimized viral proteins using pET28a expression system. Even though I have the perfect insert in the recombinant plasmid, there was absolutely no...

24 September 2020 3,635 8 View

Non fluorescent E. coli GFP?

I Have a problem with E. coli GFP, I am culturing them in LB medium/ampicillin + IPTG, but not all the cells fluorescent after UV excitation (fluorescence microscope) although they grew in the...

15 September 2020 4,636 1 View

How are parents guiding their children with learning difficulties?

Parents' educational level is essential in raising the level of education

14 September 2020 6,306 4 View

Is it possible to integrate ratio measures with ordinal survey scale?

24 June 2020 205 1 View

X-ray Experiments for antibody S309 Zero ini on COVID-19 ?

18 May 2020 3,666 6 View

Why encrypt Bloom filter and query in a different way in the following paper in searchable encryption?

I was read in paper in searchable encryption that encrypt bloom filter index in the following way: 1- generate secret key to encrypt bloom filter and query is: the secret key sk (M1, M2, s ),...

03 March 2020 1,448 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Apart from Nanosilica and CNT's what are the other nanomaterials readily available to use in concrete mixture?

Nanotechnology in concrete materials

01 March 2021 3,379 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View