I want to check mature rno-miR-214 level in rat cells.I want to order TaqMan MicroRNA™ Assays kit. how can i get miR214 specific RT primer for cDNA synthesis? how to place order for primer and probe?
secondly i have Roche Light Cycler 480 ll machiene does taqman probes are compatible for this machiene? and i dont want to order Universal MasterMix without UNG (P/N: 4324018, Applied Biosystems) which is the alternative for this kit?
My lab does not have any set up for real time PCR, Can you reccomend what are other requirements for mature rno-miR-214 detection
Hello Rajashree
I have worked with applied Applied Biosystems (AB) in my research in miRNAs with good results. When you make to order. The format of primers from AB is right to get cDNA synthesis and PCR reaction.
Regards
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