MAPK has a significant role in signal transduction. why it is produced with a cascade instead of producing directly?

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

Can I Use D-PBS for Preparing a 10% Sucrose Solution for Lentivirus Concentration?

Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...

21 July 2024 4,676 4 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Lentivirus transduction of HEK293T cells?

i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...

07 July 2024 2,946 1 View

Can anyone send me the detailed protocol of lentiviral titer determination and its invitro transduction into Hela cell line or MSCs?

I have searched a lot of information from the published article but couldn't find valuable. As I have prepared the Lentiviral vector and stored it in -80 degree!

21 June 2024 3,484 1 View

What is the difference of one-port and two-port data in the radar signal processing?

For the radar signal processing, we can use either one port signal (ex. S11) and two port signal (ex. S21). What is the difference or pros and cons of the two methods?

20 June 2024 6,052 1 View

Optimal conditions for lentiviral transduction of B-ALL cells (I'm using Nalm6 cells)?

Dear Esteemed Researchers, I am currently waist-deep into the literature to determine the optimal cell density and transduction volume for lentiviral transduction conducted in 24- and 48-well...

10 June 2024 4,167 0 View

Lentiviral Transduction Issues?

Hi there, I am attempting to transduce cells with the TLCV2 plasmid containing our sgRNA of interest. My transfection of the HEK 293T cells seems to yield positive results as the EGFP is clearly...

06 June 2024 2,131 1 View

What does effector:target ratio really means in CAR-T cell therapy?

Regarding CAR-T cell therapy, during co-cultures with different effector:target ratios, when we refer to "effector", does it involve the total T cells? For example, does a ratio of 5:1 mean 5...

28 May 2024 2,757 4 View

How to transduce lentiviral vector into ThP1 macrophages and Mesenchymal stem cells?

Is there any authentic reference from where I can get appropriate information regarding this and anyone who has experienced any one of these cells' transduction?

27 May 2024 6,554 0 View

Manash K. Paul

I think MAP-kinases can regulate multiple pathways and different downstream kinases have specificity to activate different pathways differentially depending on the cellular availability of substrates. A cascade also allows differential inhibition of each component. These are like safety valves in the system at multiple places in the circuit to have better control of the cellular system.

Nurefşan Cirik

thank you for your clear answers Cornelius Krasel Manash K. Paul