Why is Patch Dock returning my docking search?

the Patch Dock is giving me trouble regarding the code for my ligand the error message is " Error in receptor molecule input: please specify PDB code or upload file " My ligand is microRNA. and it...

05 June 2019 5,086 3 View

Is Lambert Academic publishers worth the try ?

Attached below is the second message i received from Lambert Academic publishing. although the offer is kind of enticing but i do not want to jump into less reliable publishers. Please assist...

08 September 2018 7,726 0 View

Trouble shooting qPCR ?

Any best way to troubleshoot qPCR, working currently on qPCR but when the gel is run, all i see is shadows and no distinct band, What variable should i change and also is two parameters...

04 May 2018 8,558 0 View

What database can i find the expressions of colorectum as a tissue ?

Dear all, I want to extract the genes related to colorectum but the database TIGER only showed the tissue view of colon. please suggest any database that can give the ESTs or expression profile of...

03 April 2018 1,056 8 View

What is text-mining in Bioinformatics ?

Can BLAST and CD-HIT be regarded as text mining process in bioinformatics or are there special or specific tools used to text mine data ?

03 April 2018 3,907 0 View

Limited candidate miRNA, how do i increase my search for data mining ?

Hello all, i am trying to compare the results of blast to cd hit est 2d. the initial run gave 15 and 10 respectively but the common miRNAs between them is 6. I want to increase the miRNAs...

03 April 2018 1,826 2 View

Where can i learn BioInformatic tools?

I am new to Biotechnology and my area of PhD research now is Bioinformatics. I find it very hard to run smoothly most of the software given to me to carry out biology result. Can anyone suggest...

03 April 2018 5,121 4 View

Which online target prediction tool is best for predicting miRNA targets considering a friendly interface ?

I need to identify some targets of a number of miRNAs but i need suggestions on the best target prediction tool or a combination majorly for humans. Thanks

03 April 2018 7,020 3 View

What's the best cell-lines to be used when studying stem cell ?

In invitro studies, is it feasible using neuronal stem cell model to investigate the mechanism of how anti-psychotic drugs affect neuronal development, can this group suggest any previous work...

09 October 2017 9,992 3 View

The exact principle of a glucometer ?

31 December 2016 2,723 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View