How do i sequence DNA manually using Sanger's method, a protocol will help. thanks. A full procedure is highly needed
the Patch Dock is giving me trouble regarding the code for my ligand the error message is " Error in receptor molecule input: please specify PDB code or upload file " My ligand is microRNA. and it...
05 June 2019 5,197 3 View
Attached below is the second message i received from Lambert Academic publishing. although the offer is kind of enticing but i do not want to jump into less reliable publishers. Please assist...
08 September 2018 7,852 0 View
Any best way to troubleshoot qPCR, working currently on qPCR but when the gel is run, all i see is shadows and no distinct band, What variable should i change and also is two parameters...
04 May 2018 8,766 0 View
Dear all, I want to extract the genes related to colorectum but the database TIGER only showed the tissue view of colon. please suggest any database that can give the ESTs or expression profile of...
03 April 2018 1,170 8 View
Can BLAST and CD-HIT be regarded as text mining process in bioinformatics or are there special or specific tools used to text mine data ?
03 April 2018 4,045 0 View
I am new to Biotechnology and my area of PhD research now is Bioinformatics. I find it very hard to run smoothly most of the software given to me to carry out biology result. Can anyone suggest...
03 April 2018 5,246 4 View
I need to identify some targets of a number of miRNAs but i need suggestions on the best target prediction tool or a combination majorly for humans. Thanks
03 April 2018 7,137 3 View
Hello all, i am trying to compare the results of blast to cd hit est 2d. the initial run gave 15 and 10 respectively but the common miRNAs between them is 6. I want to increase the miRNAs...
03 April 2018 1,953 2 View
In invitro studies, is it feasible using neuronal stem cell model to investigate the mechanism of how anti-psychotic drugs affect neuronal development, can this group suggest any previous work...
09 October 2017 10,110 3 View
Does gluconic acid react with ferricyanide or Ferricyanide is used as an electron mediator between the enzyme and the electrode. ? or can someone clearly explain the principle or refer an article
31 December 2016 2,921 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View