Why is Patch Dock returning my docking search?

the Patch Dock is giving me trouble regarding the code for my ligand the error message is " Error in receptor molecule input: please specify PDB code or upload file " My ligand is microRNA. and it...

05 June 2019 5,086 3 View

Is Lambert Academic publishers worth the try ?

Attached below is the second message i received from Lambert Academic publishing. although the offer is kind of enticing but i do not want to jump into less reliable publishers. Please assist...

08 September 2018 7,726 0 View

What database can i find the expressions of colorectum as a tissue ?

Dear all, I want to extract the genes related to colorectum but the database TIGER only showed the tissue view of colon. please suggest any database that can give the ESTs or expression profile of...

03 April 2018 1,056 8 View

What is text-mining in Bioinformatics ?

Can BLAST and CD-HIT be regarded as text mining process in bioinformatics or are there special or specific tools used to text mine data ?

03 April 2018 3,907 0 View

Limited candidate miRNA, how do i increase my search for data mining ?

Hello all, i am trying to compare the results of blast to cd hit est 2d. the initial run gave 15 and 10 respectively but the common miRNAs between them is 6. I want to increase the miRNAs...

03 April 2018 1,826 2 View

Where can i learn BioInformatic tools?

I am new to Biotechnology and my area of PhD research now is Bioinformatics. I find it very hard to run smoothly most of the software given to me to carry out biology result. Can anyone suggest...

03 April 2018 5,121 4 View

Which online target prediction tool is best for predicting miRNA targets considering a friendly interface ?

I need to identify some targets of a number of miRNAs but i need suggestions on the best target prediction tool or a combination majorly for humans. Thanks

03 April 2018 7,020 3 View

What's the best cell-lines to be used when studying stem cell ?

In invitro studies, is it feasible using neuronal stem cell model to investigate the mechanism of how anti-psychotic drugs affect neuronal development, can this group suggest any previous work...

09 October 2017 9,992 3 View

The exact principle of a glucometer ?

31 December 2016 2,723 0 View

Is there available rewiews on the modified flesh eating bacteria ?

although it is not well known to the world about Necrotizing fasciitis but the modified form of this bacteria can be a good anti cancer agent. are there present work or reviews on this ?

07 August 2016 8,761 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View