Any best way to troubleshoot qPCR, working currently on qPCR but when the gel is run, all i see is shadows and no distinct band, What variable should i change and also is two parameters appropriate to be varied at ones ?
the Patch Dock is giving me trouble regarding the code for my ligand the error message is " Error in receptor molecule input: please specify PDB code or upload file " My ligand is microRNA. and it...
05 June 2019 5,197 3 View
Attached below is the second message i received from Lambert Academic publishing. although the offer is kind of enticing but i do not want to jump into less reliable publishers. Please assist...
08 September 2018 7,852 0 View
Dear all, I want to extract the genes related to colorectum but the database TIGER only showed the tissue view of colon. please suggest any database that can give the ESTs or expression profile of...
03 April 2018 1,170 8 View
Can BLAST and CD-HIT be regarded as text mining process in bioinformatics or are there special or specific tools used to text mine data ?
03 April 2018 4,045 0 View
I am new to Biotechnology and my area of PhD research now is Bioinformatics. I find it very hard to run smoothly most of the software given to me to carry out biology result. Can anyone suggest...
03 April 2018 5,246 4 View
I need to identify some targets of a number of miRNAs but i need suggestions on the best target prediction tool or a combination majorly for humans. Thanks
03 April 2018 7,137 3 View
Hello all, i am trying to compare the results of blast to cd hit est 2d. the initial run gave 15 and 10 respectively but the common miRNAs between them is 6. I want to increase the miRNAs...
03 April 2018 1,953 2 View
In invitro studies, is it feasible using neuronal stem cell model to investigate the mechanism of how anti-psychotic drugs affect neuronal development, can this group suggest any previous work...
09 October 2017 10,110 3 View
Does gluconic acid react with ferricyanide or Ferricyanide is used as an electron mediator between the enzyme and the electrode. ? or can someone clearly explain the principle or refer an article
31 December 2016 2,921 0 View
although it is not well known to the world about Necrotizing fasciitis but the modified form of this bacteria can be a good anti cancer agent. are there present work or reviews on this ?
07 August 2016 8,879 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View