Cell Culture Protocol for 67NR and 4T07 cells?

Hello there! My lab has recently been (very graciously) gifted an array of cell lines, of which include 67NR and 4T07 cell lines. While I've found a decent amount of background and culturing...

02 February 2021 2,590 3 View

How can I calculate Standard Deviation using DataWarrior?

I am analyzing about 500 results in DataWarrior and I need to calculate the mean value and the SD. I managed to calculate the mean value using excel, but it I couln't find a way to simply...

25 October 2020 9,318 3 View

Computer vision / machine learning techniques for "blob" tracking and prediction?

My research involves tracking a dye plume in the coastal zone using computer vision techniques, and potentially making predictions using machine learning. If anyone knows any resources that come...

15 April 2020 6,507 2 View

Escalating dose regimen with osmotic mini pump?

Hi everyone, I wonder if someone has ever used osmotic mini-pump for an escalating dose regimen. I read that some iPRECIO osmotic mini-pump are programmable and therefore can be used for infusion...

03 April 2020 9,009 3 View

Is there anyone that have a trasfection protocol optimized for suspension cell culture?

We work with WIL2 cell line and want to trasfect mirna mimic and inhibitor. We tried first with lipofectamin and than electroporation (using amaxa nucleofector, U-001 program), none of that works....

27 March 2017 4,176 4 View

IWP-2 inhibitor

16 February 2016 1,730 4 View

Is there any solvent to solve SEBS blend?

I need to know the amount of oil present as plasticizer, so I would like to know il i can dissolve SEBS in one solvent and then recrystallyze it in another solvent.

30 September 2015 9,013 2 View

Could you help me with a Capitellidae identification?

Hi, I'm working with samples of the Portuguese Continental Shelf; I found few specimens of Capitellidae with 11 thoracic segments, in which the first is achaetigerous and the other 10 with only...

19 July 2015 1,087 4 View

Is anyone working with PK15 cell line free form circovirus?

I would like to find PK15 cell line free of Circovirus to attempt PCV2 isolation.

06 May 2015 6,617 2 View

Does any body explain why there is a superheater stage in the organic rankine cycles (ORC)?

I am interested in ORC-system. If I look to the hp-diagrams of the main working fluid they do not need a superheating stage, but in reality I saw that in many ORC the fluid is also superheated....

18 February 2015 10,018 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View