What should the village government do in dealing with the spread of the covid-19 virus?

I am currently researching village government policies regarding the spread of the covid-19 virus, I want to gather opinions from various villages or areas affected by the Covid-19 virus, what...

07 September 2020 303 5 View

What are the importance of genetic tests to detect cancer?

Cancer is not a single disease with a single type of treatment. There are more than 200 different types of cancer, each with its own name and treatment. Medical tests can look for many inherited...

21 March 2019 209 5 View

How can PLGA be solidified while making composite with hydroxyapatite?

Hello people, I have been trying to develop composites from PLGA (Molar ratio- 50:50) with hydroxyapatite by solvent casting technique. But the slurry formed by mixing the two components does not...

27 December 2018 8,313 2 View

Why there is a decrease in the intensity and merging of xrd peaks of Hydroxyapatite after soaking it in SBF solution for 28 days?

I have prepared Hydroxyapatite pellets and immersed it in SBF solution for 28 days. The xrd analysis of post SBF Hydroxyapatite shows decrease in the intensity of peak and merging of some peaks...

20 November 2018 4,271 10 View

What are the challenges and outlook of biomass utilization in Metallurgical Industries?

One of the great challenges is to find a sustainable way to obtain biobased products from renewable resources. A significant emphasis has been on the developmental biorefinery concept and...

28 July 2018 2,346 3 View

How to calculate residual stresses for graphene using Raman sprectroscopy ?

After obtaining Raman spectroscopy of Graphene before and after composite sintering. The residual stresses in graphene to be calculated.

22 June 2018 1,863 3 View

What are the new improvements that make LED a powerful tool for lighting systems optimized for photosynthesis?

24 November 2017 9,935 5 View

HI, I need help to analyze the data obtained by in vivo recording of spontaneous activity from locus ceruleaus of mouse brain.

I would like to know in detail (step by step) how to analyze the data for spike detection and sorting using Axograph. Please find the raw data as attached. Thank you very much ! Please do to...

30 July 2017 6,075 1 View

How to identifying & charecterization of lactobacillus & Bifidobacterium? ??

25 July 2017 9,836 2 View

Bifidobacterium ::: how to isolate the colonies from capsules ?

17 July 2017 3,536 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Alternatives to .45 um filter to isolate MS2 from lysed E. coli?

Hello, I am looking for alternatives or cheaper materials to isolate out MS2 from E. coli following lysis via chloroform. Some literature I have reviewed uses a .45 um nanopore filter following...

02 February 2021 807 3 View

What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View

Recommendations for a microbial strain repository?

I'm looking for an open repository or culture collection to deposit some genetically engineered E. coli strains (from the papers below). The primary goal is to preserve those strains for the long...

11 January 2021 4,398 1 View

Normalisation of qPCR between two different species?

If I have a gene carried on a plasmid and the plasmid is transformed into two strains of different bacterial species (Salmonella and e. coli). If I want to do RT-qPCR to quantify the expression of...

03 December 2020 8,051 5 View

Is e.coli ok at Electroporation at 30C?

For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...

02 December 2020 7,133 5 View

What cut-off should we use when applying BUSCO to Escherichia coli (Bacteria) genome assemblies?

We would like to analyse thousands of E. coli genomes and identify orthologous genes. Before this we would like to filter the bad quality genomes by BUSCO (https://busco.ezlab.org/). What % of...

15 November 2020 5,152 3 View

How to clear cell lysates in 96-well deep well plates?

I am expressing a construct in E. coli under different conditions to check soluble expression. I have a large array of conditions, which cannot be done in flasks. Thus, I will be growing cultures...

01 November 2020 2,716 6 View