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Dear all
Recently, I made an experiment and produced a question. When using quantitative sar to produce dem, does this dem reflect the terrain of the master image or the terrain of the slave image?
I look forward to your reply.
Thanks.
I ran a SDS-PAGE of my sample ovalbumin in histidine buffer in 2x Laemmli buffer without heating and it got nice visible bands. However, when I heat the sample at 77C in 2x laemmli buffer for 5...
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Hello everyone, I am currently working with targeting proteins to yeast mitochondria. Our strategy is to use a mitochondrial targeting signal to transport our protein of interest to mitochondrial...
16 October 2019 6,572 5 View
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I have attached a picture for my most recent western blot. My Beta-actin always shows up completely perfect but all other protein blots appear gray and messy. This has happened multiple times....
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Hi all, I am trying to generate some stable cell lines expressing either the WT or mutant version of my gene of interest (GOI). My lentiviral construct consists of a BSD gene for blasticidin...
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I performed a cell invasion assay to asses invasive capability of two different tumor population by using matrigel coated inserts. After experiment, I found that: No. of stained cells on the...
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After treating a compound to my cells, I noticed that cells died in a way I have never seen before (see the attached image - panel A). I don't think its apoptosis (apoptosis look like panel B)....
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I have selected brain tumor images ...but now found that already lots of research done n this topic.
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i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).
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Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru
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Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
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I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
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Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...
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Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...
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Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...
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Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
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