I am looking for a protocol for a macrophage killing assay using bacteria. Could someone suggest me some links or something?
Thanks a lot
Dear Mantica
Look PDF attached I hope help you
Good luck
Dear Awatif,
Thank you very much!
If anyone else has more protocols or articles please let me know!
try to use Gentamicin protection assay, http://www.bio-protocol.org/e1235
Nawazat: thank you!
An article in 1994 (Drevets et al.) demonstrated that Gentamicin can accumulate into macrophages via pinocytosis and, even if in small concentration, can impair the precise assessment of phagocytosis.
yes, the bacteria will be found within the phagosomes and this will protect them from the added gentamicin, besides the antibiotic needs time to get the bacteriocidal or static concentration within the cells
Dear Giovanna,
You may be interested in our studies of phagocytosis using different test objects are of bacterial origin, which has the advantage over the inert particles, allowing to estimate, as absorption and digestive ability of phagocytic cells (neutrophils and macrophages) as well as opsonic activity of serum and other humoral factors of the host’s innate immunity. Some own changes in the phagocytic tests and the levels of phagocytosis as an important defense mechanism of innate immunity in different physiological (including ageing) and pathological processes (inflammation, burns, etc.) the use of different biomodels, including animals gnotobiotes, was the subject of our studies in previous years.
Sorry, that some of the material is partially available in Russian only.
Good luck!
Prof. Dr. Gennady Podoprigora
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