Serum role in opsonization is host specific which means use the mice sera for mice macrophages otherwise it be having the inhibitory effect on phagocytosis, I was working on mycobacterial interaction with host cells and looked at role of different sera in the bacterial invasion and found that the host sera is host specific
Am afraid you can not do so, because of high sensitivity of complement components in work, hence you can not use different species specific monoclonal antibody for complement detection moreover different epitops.