Hello,

I have been trying to optimize a protocol for in vivo X-ChIP using a H3 Ab and I have been getting rough pulldown numbers of like 0.2%. I realize that with the tissue being embryonic there might be less pulldown than adult tissue, but this still seems awfully low. I plan on keep testing longer and longer fixation times until the shearing is negatively affected just to see if I'm not fixing long enough, but does anybody have any other suggestions? I'm using an abcam ChIP-grade Ab FWIW