Hi, good day...I conducted a human study (randomised crossover intervention trial), but when I performed glutathione (GSH) analysis on whole blood I am getting very very low GSH value (25 - 260 umol), my supervisor said normally GSH value should be in the range of 800 - 1300..
If anyone has encountered or experienced this before, low GSH value in whole blood sample, pls help on the possible causes or remedies?
Thanks...
Dear Viety,
Can you please share the protocol which u have followed to determine the GSH level?
Secondly, it is not clear the concentration of GSH what you are getting. You have mentioned only the values i.e. " GSH value (25 - 260 umol)". Kindly check the concentration i.e. 25 - 260 umol / mL or L ?
With best wishes and regards,
Koner
Dear Koner
Thanks for your respond....I am using a GSH recycling method (based on enzymatic recycling of GSH using glutathione reductase [GR]) as described by Asensi et al. (1999).
Concentration is expressed in umol/mL.
Thanks
Best Regards
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