Dear all!

I do RIP experiment on Arabidopsis (antibody free, using himeric protein and coated magnetic beads). In my case I obtain as little as 1 ng of RNA from the complex.

For library preparation we have Truseq stranded total RNA (the amount should be >100 ng of total RNA). Or NebnextUltra II (which starts from 500 pg of gDNA)

We also have kit for pre-amplification of mRNA Mint.

How to be with this little amount and kits that I have?

I only see this resolution: to pre-amplificate my RNA using Mint(1-2 cycles) - fragmentate it - use Nebnext kit.

What do you think?