Low signal - Circadian luciferase assay in cell culture?

Hi, we are running circadian luciferase assays in cell lines (u2os and 3t3) driven by two different circadian promoters. We're reading the assay in a TECAN m200 luminometer using a black clear...

01 November 2018 7,642 1 View

What statistics should I used for Likert Scale?

Was wondering if anyone could help me, I am in my third year and I'm in dissertation hell. I have used 5 point Likert Scales in my questionnaire (Strongly agree, agree, so on). I'm now looking...

19 January 2018 6,122 5 View

Another question on transcription and coding, can anyone provide up to date help?

Hi everybody, I'm undertaking my first qualitative research and am somewhat overwhelmed by the (general consensus) of time it will take to transcribe qualitative interviews - I am intending on...

10 October 2017 3,011 3 View

How can I reduce Basal Activity with TetR systems while maintaining "tunability"?

25 July 2014 3,316 2 View

Troubleshooting protein purification?

Bound clarified lysate for 1hr @ 4C in 150mM Tris, 50mM NaCl, 40mM Imidazole.

23 February 2013 5,194 17 View

Microscale MudPIT without 2D-LC system?

After concentrating the secretome (about 5 micrograms of sample) from conditioned media sample and preparing a microscale digest (directly using organic cosolvent with no detergents or salts to...

30 September 2012 7,798 10 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View