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29 January 2021 2,623 1 View

TdT vs. PAP for polyadenylation?

Hello, From my understanding, there are two ways to add polyA tails to RNA. 1) with polyA polymerase (PAP) and ATP to RNA or 2) with terminal transferase (TdT) plus dATP and ddATP to ssDNA...

18 October 2020 6,078 2 View

How to perform western blot for Toll-like receptor 3?

I encountered some problem to detect TLR3 for my western. I did not get any bands. After reading a bunch of literature, I am not sure exactly what I did was wrong. I appreciate if anyone has...

19 July 2020 9,402 3 View

Nature of Racism?

I am writing a scholarly advocacy article - the question is: "What is the problem with using an historically specific example of racism as a template for understanding the multifaceted nature of...

29 May 2020 7,848 22 View

What effect size calculation to use for meta-analysis?

I am conducting a meta-analysis for the association between TNBC and BRCA1 and BRCA2. Can anybody help me as to what 2x2 frequency table to use- standardised mean difference, correlation...

05 March 2020 4,992 3 View

Have I got the settings right?

I want anyone to be able to download my paper "elderberry is antibacterial, antiviral etc" for free and easily. Have I got the settings right? With Covid-19 spreading I think elderberry (Sambucus...

02 March 2020 6,607 3 View

I'm looking for a measure of self-perceived attractiveness for a study I'm conducting at my university. Does anyone know of an existing measure?

I've looked around for a survey for this measure, but I can't find one anywhere. I originally was just using a universal 10-point rating, but my professor would like to see something more...

01 March 2020 6,529 3 View

GFP/RFP constitutively expressed from integrated transgene in E. coli?

It would be very useful to have tame strains of E. coli that are always fluorescent. So far the only ones I can find are from this work: Pinheiro, Bergquist et al. (2008), Applied Microbiology and...

09 February 2020 9,918 2 View

How many E.coli cells should I sort via FACS for RNA-seq?

Hello, I have been trying to find studies that have FACS sorted E.coli in bulk for RNA extraction and RNA-seq. Maybe I am bad at lit-searching, but I have been surprised to not find a clear...

14 November 2019 4,668 3 View

Preferred mRNA quantification from prokaryotes?

Hi, I am trying to put together a list of the methods used to determine how much mRNA you have after RNA extraction. My plan is to compare total RNA vs. mRNA amounts before and after rRNA...

25 April 2019 7,971 2 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View