What are examples of data-intensive research leading to totally new insights?

Data-intensive research is still all the rage these days. Breakthroughs in AI (and especially NLP) seem likely to only accelerate its adoption. But can anybody provide examples (preferably from...

17 January 2021 10,008 4 View

Can somebody recommend a dual expression vector?

09 January 2021 2,679 2 View

How to merge two Bold runs (rsfmri)?

Dear all, I have 2 Bold runs of a resting state MRI of the same subject. I want to combine these two into a single bold run. I habe tried so far to use fslmerge by time, but it seems not to be...

23 October 2020 4,480 1 View

How to estimate sample size for mixed ANOVA with several factors?

I would like to estimate the sample size for a 3b x 2b x 2w x 3w x 6w ANOVA [b=between subject factor; w=within subject factor]. I do not know much about the estimated effect size or...

16 July 2020 9,457 3 View

Where can I find information to justify the crosslinking of an antibody?

Hi all, I recently did a project where I did carboxyl-to-amine immobilisation with a monoclonal interferon gamma antibody (human ELISA development kit, Mabtech). I had carboxyl groups on a carbon...

30 May 2019 6,335 7 View

Are there any studies on the effects of the duration of short rests (breaks) in work on VDU?

need some valid results on the effects of short breaks while working with the computer and display.

20 June 2018 809 4 View

Is there a Software to depict multi-channel linguistic data transcripts?

Related to my PhD-research I am looking for software that is capable of displaying multi-channel transcripts of linguistic data. More precisely, I do have video data, that includes writte (chat)...

25 February 2018 5,263 2 View

Reverse transcriptase with 5'3' exo activity

Hi! I am looking for a reverse transcriptase that has no strand displacement but rather 5' to 3' exonuclease activity. The closest thing I found is Bst polymerase from NEB but that is only a weak...

24 July 2017 6,312 2 View

Is it allowed to conduct an ANOVA with a within-factor that has no variability?

Dear folks, does anyone know whether it is allowed to calculate a repeated measures ANOVA where one factor has no variability? E.g. I have 3 types of errors (factor categories: A,B,C) and 6 action...

24 July 2017 662 3 View

How to deal with strange AIC and BIC values in a latent class analysis?

19 June 2017 9,674 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View