Hello everyone,
I'm searching for beginner-friendly books to help me navigate the realm of protein mass spectrometry. Do you have any recommendations?
You can start with Wilson and Walker proteomics chapter. It is very basic and easy to understand
Hey everyone! I'm currently delving into computational protein engineering and protein docking and would love to explore some friendly and accessible literature and methods books on the subject....
08 April 2024 1,643 0 View
Hi, I am interested to study myelinosome. I know it can be seen well in electro microscopy, however I was wondering if I could stain them with an antibody ? Thank
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An electric point charge is found at rest in some inertial frame. Another charge is accelerating along a straight line with respect to the same frame. The question is about the interaction "felt"...
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I want help on design the draw profile daily on domestic water (throughout the day) using TRNSYS. Please your help !!!
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I am wondering if anyone has used MyoD as a driver in mice (to knockdown a gene in muscle specifically) and has extracted protein, run a western blot and noticed in their KO group (when comparing...
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I am using a mitochondrial cocktail antibody on mouse muscle samples in western blot. Abcam suggested to use unheated samples as the detection of some of the complexes is sensitive to heating. Has...
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Hello everyone, I want to use gender as a covariate in an ancova. I read that I have to dummy code it beforehand (male, female, other). When doing that, I would get three variables in spss,...
22 October 2022 6,172 3 View
Dear all, radon is quite new to me and i am currently puzzled by the question if radon - as a heavy noble gas - would, in theory, tend to separate from air in a closed volume with no ventilation....
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I am bead-beating coral tissue (containing intracellular algae, bacteria, the coral animal, and a brittle coral skeleton) for up to 5 minutes (with 1-minute cooling breaks every 40 seconds) in...
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Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
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I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
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Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
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Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
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I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
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EDX analisys
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I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
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Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
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