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Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

Systematic reviews and/or meta-analysis and articles from predatory journals/publishers ?

Do you know systematic reviews and/or meta-analyses that have chosen to exclude articles from (or suspected to be) predatory journals/publishers in their work ?

24 March 2024 4,941 3 View

How to store insect sf9 cells?

Is it possible to keep them in -80 freezer or should we keep them in liquid nitrogen?

17 July 2023 2,846 1 View

We're looking for a complete system for producing anodized aluminum oxide (AAO) substrates from self-supported aluminum foil?

12 June 2023 3,526 2 View

Is there any free AI tool (machine learning) for the prediction of urinary tract infection based on dipstiks and sediment?

Dear members, As you know, the identification of urinary tract infection by culture is crucial for the diagnosis and treatment. However, traditional methods of culture identification can be...

04 May 2023 7,178 3 View

What company provide humanized mice?

Hi, I need humanized mouse models for flavivirus infection but I do not have the skills to produce them. Do you know of a company that can do it. Thank you very much

20 February 2023 3,058 0 View

No melting peak in DTA for an amorphous material ?

Hello everyone, I'm currently doing some DTA analysis on two phases of the same ceramic material, one is fully crystalline and the other fully amorphous. When I work with the crystalline one I...

03 July 2022 8,061 3 View

How to avoid yeast zygot clumping?

I would like to perform some microscopy experiments on yeast zygots. Unfortunetly zygots are clumping together which is not ideal condition for my experiment. I tried to add 0.1% of Tween80 with...

01 March 2022 8,606 2 View

Quantifying the uncertainty around the prediction of a logistic regression model: an available R package to estimate profile likelihood 95%CI?

Hi, I'm a fish biologist and I'm interested in assessing the uncertainty around the L50, which is the (sex-specific) length (L) at which you expect 1 fish out of 2 (50%) to exhibit developed...

21 November 2021 173 11 View

Why not cow dung manure used in lie for artificial ?

Cow dung manure is more powerful than artificial fertilizer.

19 July 2024 7,392 7 View

Is cow dung can be in used for repeated use ?

Is this organic cow dung manure now important than artificial fertilizer.if good then why ?

19 July 2024 9,052 2 View

How can I analyze thermal camera images to measure heat stress in dairy cows?

Hi, I am working on heat stress in dairy cows. In a recent experiment, we used a FLIR-T62101 thermal camera to take photos of the cows. One photo is attached. How can I analyze these images to...

30 June 2024 2,190 5 View

Could anyone recommend antibodies to TET2, TET3 and DNMT3B for bovine?

Can anyone recommend polyclonal or monoclonal antibodies for Tet methylcytosine dioxygenase 2 (TET2) Tet methylcytosine dioxygenase 3 (TET3) DNA methyltransferase 3 beta (DNMT3B) Ideally, they...

27 June 2024 3,557 1 View

Where can I find my citations for my published papers?

1- M.M.M. Ahmed, F.M. El Hag, F.S. Wahab, S.F. Salih (2001). Feeding strategies during dry summer for lactating desert goats in a rainfed area under tropical conditions. Small Ruminant Research 39...

18 June 2024 552 5 View

Why do rocket fly vertically to get to outer space instead of horizontally with reduced gravity since space is all around us?

if the earth is spherical then rockets can use less energy to get to outer space by flying horizontally till they reach outer space or even they can nose dive and enter outer space from beneath...

17 June 2024 1,378 3 View

I'm facing serious problem about sample size determination please help me?

I'm doing research in veterinary science and topic is associated with ticks prevalence and seasonal variation in cow..I have randomly selected 560 cows as sample size and studing for prevalence...

25 May 2024 3,834 5 View

For immunofluorescence of mouse embryos, is there a best way to store semi-long term?

I'm on a time crunch and have to fly back home and my return date to the lab where I'm doing my research is yet to be determined (hopefully in the next couple of months). I'm hoping I can store at...

15 May 2024 2,572 0 View

Why it is not common to use PD speed controller (for example for flying shears in rolling mills)?

I was simulating 3 types of speed controller types for flying shears. P, PI and PD speed controller. And my question again is, what is the reason for not using PD control even if it can...

15 May 2024 4,806 3 View

Immune competency study in shrimp (white leg shrimp). What are the parameters to access immune competency in shrimp? Am i to use hemolymph or hepato?

Going futher, i am runing a nutrition experiment using black soldier fly meal (BSFM) and polychaete meal (PM) as a replacement for fishmeal targeting maturation in Litopenaeus vannamei. This is...

11 May 2024 3,985 0 View