Hi everyone,
My lab is currently looking into performing mammalian cell culture in hypoxic conditions. Our treatment cannot be done inside the hypoxia chamber, therefore we are looking for an efficient method to transport the cells to the treatment station and back into the hypoxia chamber while maintaining low oxygen levels. Most of our cell culture will be done in p35, p60 and 6-well plates. Any idea is welcome.
Thank you.
Julia Benincasa
It may sound really simple or silly, but did you consider using parafilm around the plates?
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Marlene Kontcho
Hi Julia, we thought about it but are a little bit skeptically if that will be enough to do the trick.
Thanks Stefanos, i will look into this option.
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