Hello,
I have been trying to load silica nps with a drug and checking its loading efficiency using UV spectroscopy by taking a stock solution of the drug of same concentration as loaded with samples and my samples' supernatant but much to my surprise, the absorbance of the latter is coming HIGHER. (I also did TGA and it shows loading upto 10%.) I can't seem to understand this . I have repeated the experiment many times.
Any help or sharing of a similar experience will be highly appreciated.
Hello Ashish,
What is the solvent system you are making your drug solution with? With silica nanoparticles, it is best to use alcohol e.g. ethanol or methanol for the encapsulation and stir the solution for a good 24 h at 25 C (use a water bath for that). Or, if you have already tried with ethanol, you can probably try acetonitrile- ethanol mixture for that, if your drug is somewhat hydrophilic.
Let me know if that works for you.
Hello Sarabjit,
My solvent is water and the particles are mesoporous silica. I did adsorption at 37 degrees for 24 hours...could you please tell me what's the reason for 25? Yes I have tried with ethanol and it's still the same. Although acetonitrile ethanol system is news to me and I don't its a good idea for my studies.
Hello Ashish,
Can you please let me know following things?
1. What is the size of your nanoparticles?
2. What is the rpm you are using for settling your particles?
3. What is the absorption maxima of your drug?
Since the supernatant is showing more absorbance than the stock, it is possible that your particles are interfering in absorbance. You can also try and read the absorbance of unloaded particles at absorbance maxima of your drug and see if it is interfering.
Hope this helps!
Best luck.
Regards,
Swati
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