What is the right way to conjugate two acids?

How to conjugate two acids with amine linker?

18 February 2021 4,083 2 View

What is the critical deficiency limit in soil for Mn in wheat crop ? in terms of DTPA extractable Mn?

critical range for Mn in soils is 2.5-4.5 ppm for all plants as an average, but what is the exact amount of DTPA extractable Mn in soil for wheat crop

04 February 2021 4,008 4 View

Which solvent is suitable for product precipitation if reaction has been performed in toluene?

If condensation reaction happened between carboxylic acid and amine and amide is formed. The solvent for this reaction is toluene. After completion of reaction, how to collect product? It should...

03 February 2021 9,796 3 View

Why does the ghost atoms gives error in NICS calculation with wb97xd in gaussian09 ?

In a trial, I am trying to calculate NICS (0 and 1) values for benzene ring using gaussian09. I have placed the ghost atoms at the center, 1A, and 2A above the rings. On using the method/basis...

19 January 2021 3,188 3 View

Any researchers working on Mucuna pruriens with respect to tissue culture?

15 November 2020 8,675 1 View

Name of Journal UGC approved journal in the field of management ?

Hi, I need some reference of Journals which have monthly publication, in the filed of customer care/ luxury segment /consumer insights /CRM 1) Scopus index 2) UGC approved journal - India...

22 October 2020 7,769 3 View

Why my absorbance values are in negative for antioxidant assay by dpph method ?

plant extract stock is 10 mg/mL from which I pipetted 20, 40, 60, 80, 100, 200 uL and made up to 3 mL using methanol then i added 1 ml of 0.004% dpph solution (dissolved in methanol) incubated in...

02 September 2020 1,218 2 View

How to linearize a phase shifter?

Hi, I have a phase shifter design that produces phase shift with applied voltage, which follows a function that could be very closely approximated by a second order polynomial. However, the...

11 July 2020 8,967 2 View

Heterogeneity in a meta-analysis?

At what level, one should accept heterogeneity in a meta-analysis. What are possible calculation/test, if we get heterogeneity.

01 June 2020 9,396 6 View

Salicylic acid is poorly soluble in water, I dissolved in 65% ethanol but it is forming precipitation on storage. What to do? Which solvent is best?

It dissolved in 60-65% ethanol but after few hours if storage at 4 degree Celsius it is forming precipitation

19 January 2020 5,092 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View