i work on live caco-2 cells. but mostly when i go for confocal microscopy i have to wash the cells with PBS or HBSS to remove the stains used, due to this my cells gets detached and left very less attached for imaging. i tried using high as well as low cell density also, but it didnt work for me. Can anyone suggest who worked on Caco-2 cells? i uses confocal dishes for microscopy.