Could someone please recommend any good literature in bovine surgery and ruminant surgery, anything from scientific articles to textbooks?
Farm animal surgery
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Could you present the review or nowadays status state investigation of thermophysical and physico-mechanical properties of hydride zirconium (ZrH2)? Where can I find out properties data tables?
02 April 2024 2,025 0 View
Could anyone explain *how to* or give an example of receiving heat flow (watt) for the FE element? I understand that *GET command is useful like *GET, PAR, ELEM,NO_ELEM,...,SMICS but can't get...
01 April 2024 6,562 2 View
I have a question for the professionals. The essence of the problem: I fix the oligos on a plastic substrate, they play role of primers in solid-phase PCR. I carry out a one-step PCR with...
28 March 2024 9,110 2 View
Problem to model a simple (at first glance) stationary problem of thermomechanics in an elastic formulation in ANSYS Mechanical APDL. There is a simple block (brick), with spatial non-uniform...
28 March 2024 7,709 2 View
Hello colleagues! Several days ago I used to struggle with tech problem of 4d table input QV = QV(X,Y,Z,TIME) using Mechanical APDL commands script. I haven't solved it yet. 1) Standard technique...
22 March 2024 3,515 0 View
I am looking for B-H curves of GO steel M080-23DR (Domain Refined). It is customary to think that the "technical saturation" of GO steels is near 2.0 – 2.04 T. However, there are some indications...
04 March 2024 7,393 0 View
House-selling is one of the typical tasks of the Optimal Stopping problems. Offers come in daily for an asset, such as a house, that you wish to sell. Let Xi denote the amount of the offer...
03 February 2024 4,482 4 View
Colleagues, tell me which dye is optimal for staining RNA during gel electrophoresis? Which one do you use in your laboratory?
21 January 2024 6,373 4 View
I seek coauthors and colleagues from Uzbekistan, Kazakstan, or other CA countries in "climate-air-pollutant-health" research. It would be great to collaborate with individuals who share similar...
28 November 2023 8,493 0 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
Hello everyone. I cultured isolated PBMCs in Erythroid Expansion Medium for two weeks and then performed electroporation in my expanded PBMCs in order to reprogram them. The first 24h after the...
22 June 2024 346 4 View
We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...
20 June 2024 393 0 View
We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....
17 June 2024 1,649 0 View