Line on the bottom of SDS-PAGE gel?

27 June 2024 1 10K Report

Hi smart people!

I have a small curious question.

Attached are my student's gel - which looks weird as there is always a line on the bottom (near the smallest marker). This is self-made gels with 12% concentration.

Recently this always happen to the gels he ran.

This has never happened to me so I have trouble explaining it :(

Someone please enlighten me.

And any advice for the next? In our lab we always have to cast our own gels.

Kind regards,

Lieke

Lieke Widowati

Here are 2 more gels, I forgot to attach them...

I really have no idea how it can be

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