Are cloned plasmids less efficient in transfection?

Are pDNA that are cloned with the genes of interest less efficient? are there any drawbacks? Any drawbacks in relation with lipofectamine or using them with HeLa or HEK 293?

02 March 2016 1,795 6 View

Expressing GFP in HEK and HeLa. no ECL results?

I have been working with GFP that I have exprrssed in HEK and HeLa cell lines. On transfer I can only see the marker on the nitrocellulose. I cannot even see the positive control on the...

02 March 2016 574 5 View

Disadvantages of green fluoroscent protein (GFP)

11 December 2015 7,845 1 View

Anyone knows any papers where different bacteria were tested against antibiotics by disk diffusion method?

Bacteria tested against antibiotics

02 March 2015 2,133 3 View

Can anyone please tell me two old and current techniques used to diagnose scarlet fever or group A streptococcus in labs?

Scarlet method diagnostic methods

01 February 2015 9,754 6 View

Which microbes are good at forming biofilms and what morphological elements make them so?

Biofilm

01 February 2015 3,144 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View