Are cloned plasmids less efficient in transfection?

Are pDNA that are cloned with the genes of interest less efficient? are there any drawbacks? Any drawbacks in relation with lipofectamine or using them with HeLa or HEK 293?

02 March 2016 1,795 6 View

Expressing GFP in HEK and HeLa. no ECL results?

I have been working with GFP that I have exprrssed in HEK and HeLa cell lines. On transfer I can only see the marker on the nitrocellulose. I cannot even see the positive control on the...

02 March 2016 574 5 View

Limitations and failures in western blotting and ECL?

If anyone could please suggest me research papers wherein western blotting and ECL have failed to produce any results. I have been trying to express GFP in HeLA and HEK and on transfer I can only...

02 March 2016 6,138 0 View

Disadvantages of green fluoroscent protein (GFP)

11 December 2015 7,845 1 View

Anyone knows any papers where different bacteria were tested against antibiotics by disk diffusion method?

Bacteria tested against antibiotics

02 March 2015 2,133 3 View

Can anyone please tell me two old and current techniques used to diagnose scarlet fever or group A streptococcus in labs?

Scarlet method diagnostic methods

01 February 2015 9,754 6 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

25 January 2021 3,972 7 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View