Vishwesh Karandikar

7 Questions 12 Answers 0 Followers

Questions related from Vishwesh Karandikar

Expressing GFP in HEK and HeLa. no ECL results?

I have been working with GFP that I have exprrssed in HEK and HeLa cell lines. On transfer I can only see the marker on the nitrocellulose. I cannot even see the positive control on the...

03 March 2016 655 5 View

Limitations and failures in western blotting and ECL?

If anyone could please suggest me research papers wherein western blotting and ECL have failed to produce any results. I have been trying to express GFP in HeLA and HEK and on transfer I can only...

03 March 2016 6,207 0 View

Are cloned plasmids less efficient in transfection?

Are pDNA that are cloned with the genes of interest less efficient? are there any drawbacks? Any drawbacks in relation with lipofectamine or using them with HeLa or HEK 293?

03 March 2016 1,863 6 View

Disadvantages of green fluoroscent protein (GFP)

I wanted to know what disadvantages GFP holds. I was also searching for research papers where they have concluded the same. Thank you in advance.

12 December 2015 7,933 1 View

Anyone knows any papers where different bacteria were tested against antibiotics by disk diffusion method?

Bacteria tested against antibiotics

03 March 2015 2,191 3 View

Can anyone please tell me two old and current techniques used to diagnose scarlet fever or group A streptococcus in labs?

Scarlet method diagnostic methods

02 February 2015 9,843 6 View

Which microbes are good at forming biofilms and what morphological elements make them so?

Biofilm

02 February 2015 3,214 1 View