Dear ResearchGate Community,
I'm currently working on the implementation of the Ligature-Induced model of Periodontitis in Rats (Winstar - Sprague Dawley). Yesterday I had my first try... however I discovered rat molars are very distal and difficult to access. I tried to use a 4-0 silk suture (3/8 circle) but found it impossible to access!
Does someone has experience on this model? I need advice or guidance on how to reach the molars.
With best of Regards,
Constanza.
Ps. Sorry for my english, sadly I am not a native speaker.
Hi, Constanza, try using the 3-0 silk (despite the greater thickness, the insertion is easier, without fraying it). The second maxillary molars and the first mandible molars are, according to our group experience, the ideal ones for beginning the process. Use two needle-holders (small ones) and insert the silk first in the distal interproximal space. The insertion movement must be firm and precise, from top to bottom, and not from one side to the other, because that usually frays the thread. The next insertion can be done in the mesial interproximal space.
Good luck!
Dear Drs. Won Young and Mariana,
Thank you very much for your insight, both were really helpful. The two needle-holders was a great tip!
So I had my second attempt today. This time I was able to access to the molars nonetheless, couldn't pass the suture... there wasn't much space. Maybe the rat I used for proof-of concept was too small (Female, 250gr winstar rat). Do you think using a bigger male might make a difference? Also, I was thinking maybe it is possible to ligate the incisors and hold the suture in place using dental resin. Have you ever tried/heard that approach? (I need to think in a Plan B periodontitis model).
Regards,
Constaza.
Unfortunately, the incisors don't have the same characteristics as molars and should not be used for inducing periodontitis model. Keep trying as I described above and you will suceed! It is hard in the beginning but it is feasible. We did it in Wistar females as described in the paper in my profile. Good luck!
Dear Mariana,
Thank you very much for your guidance and advice! I did it!!!! it was, as you said, practise, patience and keep trying until you succeed! I'm really grateful for your words and encouragement.
Regards,
Constanza.
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