ligation of bilateral common carotid artery without induce hypotension is considered model of global cereberal ischemia or not?

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Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

I need a digital Transformation validated measurement scale ?

Thanks for support

25 June 2024 4,475 0 View

Trying to make optimization for Mixer on different pdk ?

i make a design topology of mixer For my graduation project working at 28 Ghz on cadence with UMC pdk and get this result S(2,1) 3.5db but when trying to make same topology with another pdk on...

09 June 2024 9,729 3 View

How can i remove the formed water in schiff base synthesis to shift the equilibrium towards the imine?

I am trying to form some ligands and some time it necessary to remove the water form by the elimination of it from the reaction .

10 May 2024 8,899 5 View

Could you email me this paper below?

I'm interested in this paper : A Data Dissemination Algorithm Based On Maximization of Causal Path Entropy in Vehicular Ad Hoc Networks Could you email me this paper?

19 April 2024 2,940 0 View

Hello, very good day everyone. I wanted to ask how many rpm to obtain the separation of a drug sample in wastewater with activated sludge?

I need to separate my drug from a sample with activated sludge and residual water

17 April 2024 9,601 2 View

How to characterize the biogenic nanoparticles for the capping agents? How can capping agents be isolated ? Can anyone please share an article ?

Seeking a protocol to isolate the capping agents from prepared nanoparticles how to characterize them ?

01 April 2024 6,513 3 View

Why my unstained, isotype (IgG) and positive samples (U87 cells) have same histogram results?

Hi, I just want to ask regarding flow cytometry method for indirect staining of U87 cells (Glioblastoma) as I'm still quite new in using this method. My objective is to observe the expression of...

23 March 2024 8,883 1 View

How can i complete clustering analysis and FEL analysis using gromacs ?

GROMACS version: 21.0 GROMACS modification: No I complete a 100 ns MD using gromacs v21.0 Can you please provide me a step by step command lines to complete clustering analysis and Free energy...

03 March 2024 2,482 1 View

Title: Measuring Zeta Potential of Nanosheets with Unknown Refractive Index and Handling High Polydispersity Index in Gel-Based Nanosheets ?

I am currently working on measuring the zeta potential of nanosheets, but I've encountered a challenge related to the unknown refractive index (RI) of my samples. Since the exact RI of the...

20 February 2024 2,420 3 View

Can an Ischemic Buffer Alone Effectively Mimic Hypoxia in an In Vitro Cardiac Cell Hypoxia-Reoxygenation Model?

I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...

30 June 2024 1,234 2 View

How does prolonged ischemia lead to myocardial necrosis?

Comprehensive Overview of Myocardial Infarction: Etiology, E... How does prolonged ischemia lead to myocardial necrosis?

15 June 2024 6,589 1 View

What is the appropriate timeframe to undertake CABG in face of a Slow Flow phenomenon on LAD?

In the face of a Slow Flow phenomenon (SF) raised after having resorted on fibrinolytic therapy, the patient may be referred for CABG. The SF does imped the graft flow towards the targeted...

17 May 2024 4,304 0 View

How to solve “excessive deformation” error while FSI simulation in carotid artery?

I have been trying for a long time to get my 2-way FSI using a Mechanical and fluent model of an patient-specific carotid artery to work but it keeps crashing with errors before finishing the...

28 February 2024 6,093 5 View

How to identify LAD in the rat heart?

I use the LAD ligation model in rats to model myocardial infarction. I always pierce the myocardial wall blindly in the area where the LAD should be, but I don't always get an infarction. If...

22 February 2024 3,268 0 View

How can I calculate a low WSS area percentage on a stented artery wall?

Supposing we are analyzing blood flow through a stented artery. I have been simulating one strut of a stent on the artery wall as a 2D axisymmetric model (I attached an image of the geometry). If...

04 February 2024 5,905 0 View

Prevent skin graft site complications coronary artery bypass surgery?

I am looking for literature on best practices for wrapping saphenous vein graft sites during open heart surgery, prior to leaving the operating room. Who can do and what is best practice?

21 August 2023 8,833 4 View

How can I drive flow rate waveform using Womersley pressure-flow relation in an artery?

I want to simulate pulsatile blood flow in coronary artery. Therefore, I must drive flow rate waveform in different Womersley numbers using Womersley pressure-flow relation. I have obtained the...

04 June 2023 6,727 0 View

What are the fluid resuscitation recommendations in hypotensive ESRD patients?

Patients which End Stage Renal Disease often proffer a torrid time when they develop hypotension. Use of fluid in these group is very tricky.

26 May 2023 1,560 0 View

What are the main differences/similarities of dopamine, epinephrine, and dobutamine in managing hypotension in chronically ill patients?

Which one is best for non-cardiogenic hypotensive patients and which one is best for cardiogenics?

26 May 2023 6,145 0 View