How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

Transmembrane Protein 3-D Coordinate File Preparation Question?

Hello I hope you are all doing well.:) Several transmembrane Pannexin1 3-D coordinate files have been submitted to the PDB, however, I believe all are missing coordinates in some of the Pannexin1...

11 August 2020 4,875 3 View

What are the best metrics for evaluating the quality of non-spherical clusters?

Hi. I'm dealing with clustering of data were the resulting clusters are, in general, non-spherical. Some of them are not convex. What are the best internal metrics for evaluating these kind of...

27 March 2020 5,076 2 View

How can I separate hematite from bornite in a powder?

17 March 2020 570 3 View

How can I access the total number of integration points present in my model using a vumat in Abaqus ?

Hello, I'm using a vumat in Abaqus and i'm storing data associated to each Integration Point present in my model. For the time being, I scale my array to a given large size, say M(10000). I...

08 January 2020 9,622 3 View

I have tried to clone dcas9 into pET22b vector for the past 1month now. And I do'nt always have any colonies. Anyone cloned dcas9 into pET vectors?

I always have pure insert and vector DNA in the region of 1.7-2.0. I use restriction enzymes sall HF and xhol and perform ligation overnight for 16hrs and then do transformation in 10 beta...

30 September 2019 7,178 2 View

Please, has anyone worked on sgRNA labelling? I am finding it difficult to get protocols to label with alexa dye and Qds.?

How can I label single guided RNA (sgRNA) with Alexa 488 dye and quantum dots (QDs)? I will appreciate any paper recommended or detailed protocols.

30 September 2019 6,873 1 View

How do I stop TEM samples being destroyed under electron beam?

The samples that I make for TEM contain cells. I need them to be 70-90nm (no higher) to be able to see them clearly and do EDX as well. However, They begin to rip and flex under the beam even at...

19 August 2019 2,339 1 View

Correct statistical analysis for intervention study pre and post test?

I have ran a study comparing the Daily Mile and HIIT in children to see which is best for improving cardiovascular endurance (Beep test) and anaerobic power (20m sprint). Scores on both test...

29 April 2019 7,457 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View