Hi everyone!
I am extracting leaf proteins and I have a question about precipitation. The precipitated proteins are white? The precipitate that I obtain is yellow-brown and in the electrophoresis I don't observe protein bands. Thank you.
(I have used the TCA-Acetone and TCA-Acetone-phenol method)
It depends what kind of leaf it is you used for the sample? have you analyse its proximate analysis for protein content? plants should have protein content in all body part, I think the color of the precipitate doesnt always white, I did for the stomach gastric juice of fish and the precipitate color was light brown, you will need to check the protein concentration with Lowry or Bradford method.
I hope this link will help you
https://www.google.com/amp/s/www.researchgate.net/post/What_is_the_good_method_of_protein_extraction_for_plant_leaves/amp
regards
The most important here is what buffer and procedure of the sample preparation did you use for leaves.
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