Darn it. You had to go make me use them thar Google thingee:

Here are some data taken from The Hive and the honey bee 1992 ed.

LD50 for bee venom: 2.8 mg/kg body wt

Weight of venom per bee:

147 micrograms/bee

Number of stings for LD50/kg body wt: 19 stings/kg *assuming all venom is injected examples: Child of 10 kg (22 lbs) = 190 stings for LD50 Child of 30 kg (66 lbs).

Article Intravenous Single Dose Toxicity of Sweet Bee Venom in Sprag...

Agreed to Steingrimur Stefansson answer.

First of all I would start with high quality of bee venom (extracted from the bees).

Then groups of animals. 10 each group. I would start with the literature data. After the required days I would count the number of living animals.

If is is less than 5, then you have to decrease the amount of bee venom.

If it is more than 5, increase the amount.

Decrease and increase would be half of the remaining part.

like this: start with 3.2 mg/kg. if result too much, decrease to half.

next step 1.6 mg/kg. Less, then 5 died, increase. The difference between 3.2 and 1.6 is 1.6, so increase the half of it, with 0.8.

Next step is 2.4 mg/kg.

If less, then the half is die, than increase with the half, also with 0.4 mg/kg.

Next step is 2.8. if less, then 5 increase with 0.2

If more, then decrease with 0.2

If exact 5 then you have a preliminary result.

You can repeat it on larger number of animals, with this dose.

Then you can verify with life bee sting, if you wish a very exact number . quality of bee venom function.

Why are you interested on more precise numbers?