LCMS analysis, I am getting 8Da more for a protein what can be the reason ?

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Adam B Shapiro

The mass spec may be miscalibrated. If you have not done so, perform a calibration just before running your sample.

Peter Allen Faull

Hello Shamu,

The subsitiution of a Glu residue with a His residue will lead to a mass increase of 8.016319Da.

Is this a possibility for your work?

Kind regards,

Peter

Shamu Tn

Dear Peter Allen

Its not possible because I have DNA seq of construct and amino acid seq too..but a good try and thanks.

Adam B Shapiro is right