Dear Dr. Cao,
I read your interesting article. I was wondering if you could send us an aliquot of LATS1 3'UTR reporter. Please let me know.
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
I am looking for supporting studies discussing literature limitations on the relationships between digital capabilities, market orientation, brand perception and business performance. Also, a...
14 February 2021 7,457 5 View
Hello there.. I've recently submitted my master thesis in marketing. As a reseacher, I now know that the purpose of a master degree is to know academic research tools and how to do and write an...
09 February 2021 6,692 9 View
Hi In the design of a bidentate ligand with imine group, is it okay to have a 3,4,5-trihydroxyphenyl group in the structure? In other words, does the 3,4,5-trihydroxyphenyl group compete with...
08 February 2021 4,977 7 View
Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...
04 February 2021 721 6 View
Also it's effects on plants.
22 December 2020 2,318 3 View
Dear all, I have right now a problem with measuring the charge mobility of different polymers. I have built different types of thin layers OLEDs using different thickness types of polymers. In...
01 December 2020 5,673 4 View
Hello Good Greeting for everyone I want help. What moment we can measure the polarization behavior by changing the external resistance in the Microbial fuel cell during the operation? If we are...
24 November 2020 7,100 2 View
Hello I am trying to do flowcytometry for BAL fluid so i inject1ml of PBS into the trachrea and aspirate back(this done three times) . to calculate the cell count in bal i use a cell counter...
16 November 2020 2,367 2 View
I want to test chitosan nanoparticles as antimicrobial against pseudomonas How to use resazurin microtitre assay????
08 November 2020 9,533 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...
22 February 2021 2,072 3 View
Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...
16 February 2021 1,337 2 View
I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...
16 February 2021 4,224 3 View
I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...
14 February 2021 3,142 7 View
I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...
11 February 2021 1,328 3 View
Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...
10 February 2021 1,797 5 View