I need to learn lateral flow assay can someone please share the videos for different steps of this assay as are available for other tests like PCR. I am hesitant to start my assay as I feel totally blank and have just the theoretical knowldge for LFA but no hands on training. As I am out sourcing the antibodies that are quite expensive and I dont want to waste my chemicals. Therefore please share different steps of membrane assembling, blocking of membrane, making various buffers, making colloidal gold particles and dilution as well as immobilization of antibodies on membrane. I have found few videos but that are not really helpful.
http://cyberspaceandtime.com/fdWwBuS0NNw.video+related
http://cyberspaceandtime.com/fdWwBuS0NNw.video+related
https://www.research.manchester.ac.uk/portal/files/84030840/FULL_TEXT.PDF
https://cdn.shopify.com/s/files/1/0257/8237/files/BioReady_Lateral_Flow_Handbook_v-1.0.pdf
https://pdfs.semanticscholar.org/0491/153846d4cff5d4e4fbeb0bbcf67cb44a61bd.pdf
http://www.diagnostics1.com/MANUAL/LFIA_Book%5B1%5D.pdf
https://www.emdmillipore.com/Web-US-Site/en_CA/-/USD/ShowDocument-Pronet?id=201306.12550
https://reader.elsevier.com/reader/sd/pii/S131961031400129X?token=AAE566F61E69C1EB03749538C66907B109F7ACD3B2D03BE31A5D80AE3658F265668FD2D65D3FA863ED99E0F61B381635
https://nanocomposix.com/pages/lateral-flow-test-strip-assembly#target
https://www.biodot.com/lateral-flow/
https://www.youtube.com/watch?v=uHpsV3p514Q
https://www.youtube.com/watch?v=hp7kGrc8srM
https://www.youtube.com/watch?v=PCP8KUVjJoo
https://www.youtube.com/watch?v=tg8gm5HkGc0
Dear,
just buy pregnancy test from market. and do some trial on that test, so0 you will get the basic detail.
Pregnancy test is cheaper.
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