Hey,
I would like to dissociate Langerhans Islet to obtain single cell,
I know that it is possible with Trypsin but at which concentration and for how long (approximately) ?
Tanks for your help,
Tom
… with my present female lover … she and I spend anywhere from two hours to six hours in caressing, touching, cuddling, hugging, lip kissing, deep kissing and intimate conversation before,...
11 August 2024 4,521 0 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
Your partner’s not there to service you, it’s not their job to keep you sexually satisfied. You’re together because you love each other and want to make each other happy. Constantly hassling them...
08 August 2024 1,491 0 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?
04 August 2024 1,078 1 View
Women often complain that their husbands never touch them unless they want sex. (Michele Weiner-Davis)
02 August 2024 7,778 2 View
Women need to feel a degree of sexual intimacy before sex becomes desirable… For women, intimacy sometimes results in sex; for men, sex sometimes results in intimacy. (Marina Muratore)
31 July 2024 8,860 0 View
Women associate affection with love. … Men associate affection much more directly with sex. … Men see affection of any kind as a sexual invitation. Many women find this bewildering. (Kramer &...
30 July 2024 9,498 2 View
If the pairs are similar, will it reduce the adhesion due to electric charges?
29 July 2024 3,185 2 View
Women also often find it easier to fantasise when self-pleasuring than in sex with a partner. The immediacy of someone else’s needs actually inhibits the expression and satisfaction of their own....
26 July 2024 8,351 2 View
I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...
22 July 2024 412 3 View
I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...
26 June 2024 3,664 1 View
Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...
22 June 2024 1,124 5 View
Hello, I got reviver comment " please look into the comparative assessments regarding Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant" of the target...
09 June 2024 10,069 3 View
We produced scFv of an antibody from bacteria. Now we want to establish dissociation Kd of antibody using quantitative elisa. How can i know the expected Kd value (range in nM, or mM)?
04 June 2024 2,602 5 View
We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....
28 May 2024 6,052 2 View
At the risk of asking a stupid question. I'm planning to start isotropic fractionator experiments on insects. The protocol says to make a dissociation solution of 1L of 1% triton x-100 in 40mM...
27 May 2024 821 2 View
When I calculated Kd, I found that although the concentrations were set to satisfy the type of binding used to determine the differential spectrum, Kd was unstable if the two high concentrations...
14 May 2024 399 0 View
Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...
07 May 2024 4,706 0 View
I used Microscale thermophoresis(MST, from Nanotemper) to determine the constant of dissociation (Kd) between a protein (20 nM) and a ligand (1:2 serial dilution from 50 μM to 1.5 nM). I ran the...
04 May 2024 2,066 0 View