Bacteriophages adhesion process with examples.
Dear colleagues, I have spent a few weeks trying to figure this problem out with no success, so that I post this question here. First of all, I have to say I am new to LBM method. I am working...
11 June 2024 1,084 3 View
Hi to all, Just a question, knowing the brines origin of the gypsum and having found pegmatitic clusters of gypsum, with crystals up to 10-12cm, and knowing the ionic interactions of the elements...
13 May 2024 3,657 0 View
Hello everyone, I'm trying to obtain curves of friction coefficient as a function of entrainment speed using a tribology setup geometry in an Anton Paar rheometer. However, the software gives me...
01 May 2024 4,089 2 View
I need to know what will I present on this topic
18 April 2024 563 1 View
Hello everyone! I've been having some difficulty in finding datasets for specific variables: - Dissolved oxygen - Upwelling index - Sea surface Salinity I was hoping of getting datasets of...
15 April 2024 5,354 2 View
In the calculation of free energy of solvation using GROMACS, for a molecule with negative charge (-6) and approximately 100 atoms, ¿how many lambdas are necessary to calculate the free energy...
03 April 2024 316 0 View
How do I calculate the Reaction Coordinate values in axis-x (an equation)? Example in the next image:
03 April 2024 892 0 View
Send me an e-mail for more details at [email protected]
15 March 2024 3,940 0 View
I am considering undertaking a quant + qual research by providing teachers with a questionnaire to measure their feelings and practice with regards to a type of music. I was thinking of...
14 March 2024 5,757 11 View
Dear scientists, we are currently carrying out a wound healing mouse model. The process is as follows: make the wound, apply and suture the silicone splint, put the tegaderm and apply topically...
06 March 2024 6,576 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
In the host range analysis, I saw that a temperate phage that I isolated had an effect against a different strain, but later (2 years) it did not affect that strain. What could be the explanation...
25 June 2024 9,729 3 View
After incubation of the TG1 cells with the helper phage, I spun down the culture and precipitated the supernatant with PEG/NaCl. After this, I spun it down again and resupended the pellet in PBS....
21 June 2024 6,260 2 View
Hi, I am trying to make synthetic phage library. Right now , do people still use Kunkel method or other methods? Since it looks like Kunkel method is an old method. I am looking for more...
09 June 2024 6,780 1 View
Recently, The isolation of bacteriophages from sewage water is so difficult, How to obtain phages plaques when i make isolation bacteriophage experiments for these bacterial types as bellow; 1-...
07 June 2024 9,473 1 View
Hi, During biopanning rounds of phage display, after infecting TG1 cells with eluted phages and placing them on 2xTYGA I get inconsistent TG1 colony morphology. Some rounds the colonies are...
29 May 2024 8,097 0 View
My lab doesn't use phage often and recently ran into a problem with too-low of titer for our phi 11 (to the point where I couldn't get it to lyse after 3 and 24 hours). We make a lysate prep,...
27 May 2024 7,900 1 View
I am using female BALB/c mice (6-8 weeks old, 23-25g weight) for the testing of a phage cocktail. Initially, I determined Minimum Lethal Dose (MLD) of my bacterial strain (S.aureus isolated from...
18 May 2024 6,095 1 View
