How can I activate my splenocite cells from imunized mice?

Hello everyone. We have a vaccine that elicits a protective response against a pathogen in mice but we want to know which is the T helper type that has been developed (Th1, Th2 or Th3). We want...

19 January 2020 4,549 3 View

Determining yield strength using offset requires extensometer?

I would like to know if extensometer is necessary to determine yield strength of nickel 718 alloy? Or using cross head movement to estimate the sample strain is sufficient? I'm using 0.2% offset.

13 January 2019 7,042 7 View

What is the reason for the wide price range of Carbon Nanotubes? How to assess the quality of CNTs?

Suppliers of carbon nanotubes (SWCNT, DWCNT, MWCNT) provide very different quotes for their product which, at an industrial level, should be related with their quality: - How to assess the quality...

05 December 2018 5,514 3 View

Influence of mounted sample height on SEM images?

I have several samples that have been mounted however the sample height differs, which means the working distance will differ as well. May I know how this will influence the the image captured in...

25 March 2018 7,831 4 View

What is the origin of the dots that appear in the SEM pictures of InP or InGaAsP layers after an FIB cut?

I am doing some FIB cuts and analyzing my layer stack. I always see dots in the InP and InGaAsP layers, but I don't know their origin. These dots do not appear when I do a SEM of a cleaved sample,...

27 February 2018 1,151 2 View

Meta analysis in STATA transform OR and RR into logs?

I'm carrying out a meta-analysis on STATA v.13, I'm using the effect size and CI, I transformed them into log scale but some are

03 February 2018 704 4 View

Can you help me with latent class or Latent profile analysis learning materials?

Hi, I want to learn more about latent class/ latent profile analysis as part of a research project. I wish you could point me out to materials/courses based on R or other free software. Thanks.

24 September 2017 8,355 6 View

How to assess motor skills performance?

Estimated community, I am planning to do a behavioural experiment recording fNIRS and muscular activity during (bi)manual motor tasks. These tasks are to be planned in three degrees of complexity,...

20 September 2017 6,387 4 View

The permittivity of Ag is characterized by the Drude model in Comsol Multiphysics

11 August 2017 2,606 2 View

How-to Plot Transmission Spectra vs Wavelength in Comsol

How-to Plot Transmission Spectra vs Wavelength in the Comsol Multiphysics Software?

08 June 2017 2,463 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View