Dear,

Shouqiang Cheng , You can consider the Zymo Research YeaStar Genomic DNA Kit or the Qiagen Yeast DNA Extraction Kit for total DNA isolation from yeast. If plasmid recovery is also a priority, the Zymo Research Zymoprep Yeast Plasmid Miniprep II or the Promega Wizard Genomic DNA Purification Kit may be useful. MP Biomedicals FastDNA SPIN Kit is another solid option, especially for tough yeast cell walls. Thank you!

I do not recommend the kit. The zymolysis-based method is quite efficient with some modifications.

Thanks for your response! I want to determine the copy number of a plasmid in yeast cells so I need isolate both genomic and plasmid DNA from the cell. I did not find a good commercially available kit for the experiment.

Most of kits are column based, so they might not solve your purpose. I guess below mentioned kit do total lysis and don't require comlumn for genomic dna purification. So this might be useful for you. Use chloroform cleaning for your genomic DNA, present in soup at protein precipitation step. You can repeat chloroform cleaning step to get highest quality genomic dna along with plasmid.

https://shop.biosearchtech.com/nucleic-acid-sample-preparation/dna-and-rna-purification-kits-and-reagents/masterpure-yeast-dna-purification-kit/p/MPY80200

This is the protocol I used for genomic DNA extraction from Pichia Pastoris. This should extract both genomic and plasmid DNA, however, plasmid DNA will be low. I used this method for my Pichia transformants.

Protocol for DNA Extraction from Pichia Transformants