Kindly recommend kits used for conducting Salivary B Vitamins Tests, of B6, B9, and B12?

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Tips for teaching Orton Gillingham+ to students with autism?

I am beginning to use OG+ in my district and noticed during training there are a lot of materials and routines, which may be overwhelming for my students, especially those on the autism spectrum....

30 July 2024 5,695 0 View

Does anyone know where to find polypropylene microspheres or microparticles around 1-5micrometer?

These will be used for the study of microplastics in the lung

29 July 2024 9,609 2 View

Can primary care be sustainable ? And how ?

I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...

25 July 2024 1,545 3 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View

Looking for research articles regarding effective scholarship in EBP in nursing?

11 June 2024 1,896 4 View

To what end, Ethiopia?

Ethiopia has seen escalated civil war since 2021. Immediately after Ethiopia's PM took over power in 2018, a ceasefire and a peace agreement was signed between Ethiopia and Eritrea ending one of...

06 June 2024 6,306 0 View

Random walk vs probabilistic distribution. How to use a random walk as a probabilistic distribution.?

I am looking forward to using random walks as a probability distribution for solving a particular problem in a research field. I found some blogs and articles which states different concepts of...

26 May 2024 8,302 0 View

Looking for a full text ?

I am looking for the full text of: Pfaffenrath V, Kaube H. Diagnostics of cervicogenic headache. Funct Neurol. 1990 Apr-Jun;5(2):159-64. PMID: 2227537. Hopefully someone can help me...

22 May 2024 9,624 0 View

Using a ZymoPURE II Plasmid kit, what could cause clarified lysate to turn pink and cloudy after binding buffer addition, and result in low yield?

Hi all, I used a ZymoPURE II Plasmid Maxiprep kit to isolate plasmid DNA and ended up with very low yield and poor quality. Specifically, less than 10ng/uL in 500uL from 150mL of culture, and...

15 May 2024 6,870 2 View

How to analyse WMT?

Hello, I am a PhD student and I need a help/ resources on how to clean and analyse OSPAN working memory task data! Thank you

08 May 2024 6,649 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Dear researchers, how can we download this book? Please help?

Several Complex Variables and Integral Formulas Dear researchers, how can we download this book? Please help?

22 July 2024 4,120 4 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

How can I prevent protein subunits within a protein complex from dissociating without altering the overall structure?

I have a protein complex in which some dimers easily dissociate. What methods can I use to stabilize these dimers within the complex without altering the overall structure of the complex? Will...

16 July 2024 2,757 2 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

Validated HPLC method for the estimation of the Amphotericin B ?

During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...

08 July 2024 1,330 0 View

How long can I store mouse tissues in neutral buffered formalin for, and how often should I add fresh formalin?

My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...

06 July 2024 8,360 2 View