I know that regarding the concentration of primers I've have to used 4 to 5x more and I've done that. However only one species amplifies. Any suggestions? Thanks in advance! :)
Thank you for your reply, but I'm no writing my PhD thesis and had to abandoned this idea due to the short time... however I do want do start again after delivering the thesis. So I get in touch if I need any help, I'm sure that I will :).