Anyone used Presenilin 1 [C1C2] Antibody by GeneTex?

Hello, I'm performing WB analysis using the Antibody anti-Presenilin 1 [C1C2] (cat number GTX116016) of the GeneTex company. The datasheet specifies that this antibody recognizes the full-length...

03 August 2020 7,086 1 View

How to treat mammalian diel activity patterns as a continuous variable?

Does anyone have experience in treating/converting mammalian diel activity patterns (diurnal/nocturnal/crepuscular/cathemeral) as a continuous variable rather than a factorial one? I am thinking...

13 May 2020 9,562 2 View

Any suggestions for mouse anti-phospho-ERK1/2 antibodies?

Hello, I am looking for a mouse anti-phospho ERK1/2 to perform immunofluorescence staining on cells (human iPSCs). I have experience with rabbit anti-phospho ERK1/2 #4370 from Cell Signalling...

24 April 2019 8,070 4 View

What's the dosage of Dexmedetomidine that you are using for the Drug Induced Sleep Endoscopy?

18 November 2018 1,703 1 View

DNA Output using QIAGEN Plasmid Miniprep kit?

Hi, I used for the first time the QIAGEN Plasmid Miniprep kit to extract DNA from my plasmids. After DNA extraction I've quantified the DNA concentration and the result is a DNA around 200ng/ul...

01 August 2018 5,123 6 View

RNA integrity number for urine RNA?

Dear colleagues, Do you know the minimum RNA integrity number (RNI) accepted for urine RNA in order to consider the sample for the final analysis? Any reference? Thanks, Ilaria

04 November 2017 1,320 2 View

Why by CRIPR-Cas9 I get only point mutations and how can I induce disruption of my protein of interest?

I performed CRISPR/Cas9 in CHO cells to KO the protein Arrestin3. I transfected cells with a single plasmid containing gRNA - Cas9 - YFP. Then I sorted for YFP and I screened clones by sequencing....

26 January 2017 8,704 2 View

Any suggestions on Alcian blue/nuclear fast red counterstain on paraffin slides?

Hi, I'm going to try an alcian blue/nuclear fast red staining on heart mouse paraffin slides. All the protocols that I've found in internet provide a 30' of incubation in alcian blue. Is this time...

23 April 2015 9,149 9 View

How can I measure the concentration of C60 fullerene (nanoparticles) in tissues?

I have exposed clams in vivo to C60 fullerene for 7 days. And I would like to measure a possible accumulation in gills and digestive gland. If you can help me. Thanks in advance.

17 December 2014 5,369 6 View

Could a transfected promoter be susceptible to epigenetic modifications?

I am trying to understand if the promoter that I want to stably transfect into the cells through the use of a plasmid could be epigenetically modified, as occurred for the endogenous promoter. Thanks.

26 October 2014 3,353 6 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View