It is possible to have 51 nucleotide deletion in ITS?

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Monte Carlo vs Molecular dynamics?

Hi, Imagine that you are going to sample a protein conformation (consists of N atoms) using Monte Carlo and Molecular Dynamics simulations, independently. For the MD simulation, the phase space...

06 December 2022 318 6 View

Anyone knows why the NMR integration value becomes negative?

I have two small characteristic peaks in the NMR spectrum showing negative integration values. I would appreciate it if you could share your opinion.

28 November 2022 9,933 2 View

FES diagram for Metadynamics simulation with 3 CVs?

Hi all, What is the best way to draw a FES diagram for a metadynamics simulation with 3 CVs? I know that it is still possible to draw 2D FES diagram with two CVs and project the Free energy...

11 December 2021 908 4 View

What is covenient boundary conditions for bent rib waveguide?

I want to solve a 3D wave equations in curved coordinate (for 3D bent rib waveguide) numerically by finite difference method, but I don't know about convenient boundary conditions, which boundary...

07 November 2019 3,253 4 View

What is the delivery hydrogen uptake?

The delivery hydrogen uptake was specified to the difference between H2 uptake at charging pressure (say 100 bar) and discharging pressure (say 5 bar) both at the same temperature (e.g. 77 K). But...

05 October 2018 4,608 3 View

Solvation in c++?

Dear colleagues, I'm beginner in C++ programming, I've coded a c++ program to solvate (hydrate) a structure (carbon nanotube for instance). Would you please check it out and give me your comments...

03 February 2017 7,323 9 View

What about my first program?

Dear C++ experts, I'm a beginner with C++ and I've programmed my first numerical game for training. Here, a random number was chosen between 1-15 and the gamer has three chances to guess the right...

07 January 2017 8,048 10 View

MPI error running LAMMPS?

Dear LAMMPS users I was going to run a MD simulation with about 24000 atoms on 8 threads parallel calculations, but I get the following error after 450 cycles: Process 3 exited without calling...

20 November 2016 7,656 3 View

Total energy in kcal/mol unit in LAMMPS; does it make sense?

LAMMPS computes the total energy of a single graphene sheet with 1000 atoms in kcal/mol unit. Does it make any sense?

07 December 2015 494 5 View

Could all atoms zero partial atomic charge be an option for charge equilibration (QEq) method as implemented in LAMMPS?

Dear all Could all atoms zero partial atomic charge be an option for charge equilibration (QEq) method as implemented in LAMMPS? http://lammps.sandia.gov/doc/fix_qeq_reax.html

03 November 2015 7,551 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Rasoul Zare

بله

سال 1999 در مقاله ای 20 نوکلئوتید در ورتیسیلیوم گزارش شد. ممکن است 50 تا هم باشد اگر شما مشاهده کرده اید.

ارادتمند-زارع

Valiolah Mahdizadeh

سلام آقای دکتر

ممنون، آره اون مقاله شما رو مشاهده کردم. بسیار عالی.

من ابتدا شک داشتم به اشتباه در پی سی آر و جند بار با روش های مختلف استخراج کردم دی ان ای رو و دو بار سکانس کردم. تا مطمن شم. اما یکم هنوز باور اینکه حذف 50 نوکلوتاید به طور همزمان {احتمالا قابلیت پروف ریدینگ در جهت عکس عمل کرده} در حداقل صد نسخه دی ان ای ریبوزمومی در یک قارچ و وبقای اون یکم عجیب به نظر میرسه.